Intravital Microscopy Core

The Intravital Microscopy Core

provides expert service, animal models, consultation training and intravital optical microscopy customized to the specific needs of individual kidney and urologic investigators.
develops and characterizes novel biosensor probes and in vivo delivery methods for these probes, and characterizes transgenic rat models.
further develops, characterizes and optimizes cutting edge technologies of intravital multiphoton microscopy including fluorescent lifetime imaging microscopy, and methods to increase the reach of two photon microscopy for three-dimensional imaging and quantitative analysis.
provides any of the core's developed techniques, approaches and rat strains to other investigators locally, nationally and internationally.

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Core Director

Kenneth W. Dunn, PhD

Professor of Medicine

(317) 278-0436
kwdunn@iu.edu

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Resources

Commonly used Fluorescent Probes

Animal Models in Kidney Imaging

Jove Instructional Video

Methods

Animal Request Form

Videos

Zoomed 3D volume showing a large MW dextran (green), nuclei labeled with Hoecsht (cyan), a 3kDa Cascade Blue dextran given 24hrs prior (blue), and a 3kDa Texas Red dextran infused during imaging (red). The distal tubule (center) contains filtered Texas Red dextran in the tubular lumen.

Three-D volume of a glomerulus (center) showing the capillary loops outlined by a large MW dextran (green). The nuclei are labeled with Hoechst (cyan).

A three minute time series showing infusion and rapid filtration of a 3kDa Cascade Blue dextran into proximal tubular lumens. Note the appearance of the dextran within the lumens of two distal tubules (top and bottom) at the latter portion of the time series. The brighter intensity is due to water reabsorption along the nephron and increased concentration (and fluorescence) of the dextran in the lumen.

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